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葱叶枯病菌 Stemphylium botryosum毒素的分离与除草活性研究

标题: 葱叶枯病菌 Stemphylium botryosum毒素的分离与除草活性研究
英文标题: The Study on the Isolation and Weed-controlling Activity of Pathotoxin Produced by Stemphylium botryosum
作者: 张金林,董金皋,樊慕贞,李川
英文作者: Zhang Jinlin Dong Jin’gao Fan Muzhen * Li Chuan(Mycotoxin Lab.,Agricultural University of Heibei,Baoding 071001)
出版时间: 2001-06-30
机构: 河北农业大学真菌毒素实验室!保定071001,河北农业大学真菌毒素实验室!保定071001,河北农业大学真菌毒素实验室!保定071001,河北农业大学真菌毒素实验室!保定071001
关键词: 葱叶枯病菌,毒素,分离,除草活性
英文关键词: Stemphylium botryosum,Toxin,Isolation,Weed controlling activity
刊名: 农药学学报
英文刊名: 农药学学报
ISSN: 1008-7303
期号: 02
国内刊号: 11-3995/S
基金: 河北省科委 (96 2 2 0 30 4 D)资助!项目
主题: 葱叶枯病菌 Stemphylium botryosum毒素的分离与除草活性研究
页码: 62-68
分类号: S432;
出版单位: 农药学学报
数据来源: CNKI
是否SCI: false
是否EI: false
是否核心: false
摘要: 葱叶枯病菌在活体外可以产生致病毒素 ,用改良 PS培养基培养所得滤液经TLC分离获得 、 、 、 、 5种组分 ,其 Rf 值分别为 0 .31、0 .4 7、0 .74、0 .80和 0 .90 ,在乙醇中最大紫外吸收峰分别为 2 4 0、2 4 7、2 2 3、2 36和 2 58nm。生物测定结果表明 ,组分 、 、 对马唐的生长有明显的抑制作用 ,其中以组分 生物活性最高。粗毒素对禾本科杂草种子萌发抑制效果高于对阔叶杂草 ,而对玉米、水稻、油菜等种子萌发影响很小。试验还发现 ,毒素对马唐的防效最高 ,与百草枯药效相当。毒素对马唐叶绿素 a的含量影响不大 ,但对叶绿素 b和叶绿素总含量影响较大。
英文摘要: Stemphylium botryosum could produce pathotoxin in vitro . Five fractions(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ) had been obtained by TLC and HPLC chromatography, their R f values were 0.31,0.47, 0.74, 0.80 and 0.90, and their max. ultraviolet absorption were 240,247,223,236 and 258 nm, respectively. Three fractions (Ⅱ、Ⅲ、Ⅳ) strongly inhibited the growth of Digitaria sanguinalis , among them the fraction Ⅳ had the highest activity. The inhibitory effect of crude toxin on the germination of gramineous weed seeds was much higher than that of the broad leaf weeds. This toxin has little influence on the seeds of maize, rice, rape and Chinese cabbage. The results of the greenhouse pot experiment indicated that the defensive effect of the crude toxin on Digitaria sanguinalis was the highest, equal to the effect of paraguar. Moreover the toxin could affect the content of Chlorophyll in Digitaria sanguinalis ; and especially the content of Chlorophyll b.