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免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗

标题: 免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗
英文标题: Immunoaffinity Chromatography-GC/MS for Determination of Salbutamol and Clenbuterol in Swine Liver
作者: 王建平,史为民,沈建忠
英文作者: WANG Jian-ping1,2,SHI Wei-min1,SHEN Jian-zhong1*(1.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China,2.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China)
出版时间: 2007-03-30
机构: 中国农业大学动物医学院,中国农业大学动物医学院,中国农业大学动物医学院 北京100094,河北农业大学动物科技学院,保定071000,北京100094,北京100094
关键词: 沙丁胺醇,克伦特罗,免疫亲和色谱,GC/MS,猪肝
英文关键词: salbutamol,clenbuterol,immunoaffinity chromatography,GC/MS,swine liver
刊名: 畜牧兽医学报
英文刊名: 畜牧兽医学报
ISSN: 0366-6964
期号: 03
国内刊号: 11-1985/S
主题: 免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗
页码: 67-71
分类号: S859.84;
出版单位: 畜牧兽医学报
数据来源: CNKI,WanFang
是否SCI: false
是否EI: false
是否核心: true
摘要: 制备出抗沙丁胺醇的抗血清,提取、纯化IgG制备免疫亲和色谱柱,针对沙丁胺醇与克伦特罗的动态柱容量分别为400 ng/mL基质和416 ng/mL基质。猪肝样品匀浆后用稀盐酸提取,经免疫亲和色谱柱纯化,再用GC/MS检测,即为IAC-GC/MS法。对沙丁胺醇的检测限为0.5 ng/g,定量限为2 ng/g,对克伦特罗的检测限为0.8ng/g,定量限为2.5 ng/g。空白组织按照1、5、10、20 ng/g的浓度添加药物,沙丁胺醇在空白肝中的添加回收率为78.4%-106.9%,克伦特罗的添加回收率为77.1%-102.9%。本研究建立的检测猪肝中沙丁胺醇与克伦特罗的IAC-GC/MS法乃国内首次报道。
英文摘要: An IAC column was developed by coupling the purified specific IgG against salbutamol to CNBr-activated Sephrose 4B with the dynamic capacity of 400 ng/mL gel for salbutamol and 416 ng/mL gel for clenbuterol.After extraction with dilute HCl,the extracts of swine liver homogenization was loaded on the IAC column for purification and determined by GC/MS.The limit of detection for salbutamol and clenbuterol were 0.5 ng/g and 0.8 ng/g,and the limit of quantification for salbutamol and clenbuterol were 2.0 ng/g and 2.5 ng/g respectively.Recoveries ranged 78.4%-106.9% for salbutamol and 77.1%-102.9% for clenbuterol at fortified levels of 1,5,10,20 ng/g in blank swine liver.It was the first time that the IAC-GC/MS for detection salbutamol and clenbuterol in swine liver was reported in China.