| 标题： | 免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗 |
|---|---|
| 英文标题： | Immunoaffinity Chromatography-GC/MS for Determination of Salbutamol and Clenbuterol in Swine Liver |
| 作者： | 王建平,史为民,沈建忠 |
| 英文作者： | WANG Jian-ping1,2,SHI Wei-min1,SHEN Jian-zhong1*(1.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China,2.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China) |
| 出版时间： | 2007-03-30 |
| 机构： | 中国农业大学动物医学院,中国农业大学动物医学院,中国农业大学动物医学院 北京100094,河北农业大学动物科技学院,保定071000,北京100094,北京100094 |
| 关键词： | 沙丁胺醇,克伦特罗,免疫亲和色谱,GC/MS,猪肝 |
| 英文关键词： | salbutamol,clenbuterol,immunoaffinity chromatography,GC/MS,swine liver |
| 刊名： | 畜牧兽医学报 |
| 英文刊名： | 畜牧兽医学报 |
| ISSN： | 0366-6964 |
| 期号： | 03 |
| 国内刊号： | 11-1985/S |
| 主题： | 免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗 |
| 页码： | 67-71 |
| 分类号： | S859.84； |
| 出版单位： | 畜牧兽医学报 |
| 数据来源： | CNKI,WanFang |
| 是否SCI： | false |
| 是否EI： | false |
| 是否核心： | true |
| 摘要： | 制备出抗沙丁胺醇的抗血清,提取、纯化IgG制备免疫亲和色谱柱,针对沙丁胺醇与克伦特罗的动态柱容量分别为400 ng/mL基质和416 ng/mL基质。猪肝样品匀浆后用稀盐酸提取,经免疫亲和色谱柱纯化,再用GC/MS检测,即为IAC-GC/MS法。对沙丁胺醇的检测限为0.5 ng/g,定量限为2 ng/g,对克伦特罗的检测限为0.8ng/g,定量限为2.5 ng/g。空白组织按照1、5、10、20 ng/g的浓度添加药物,沙丁胺醇在空白肝中的添加回收率为78.4%-106.9%,克伦特罗的添加回收率为77.1%-102.9%。本研究建立的检测猪肝中沙丁胺醇与克伦特罗的IAC-GC/MS法乃国内首次报道。 |
| 英文摘要： | An IAC column was developed by coupling the purified specific IgG against salbutamol to CNBr-activated Sephrose 4B with the dynamic capacity of 400 ng/mL gel for salbutamol and 416 ng/mL gel for clenbuterol.After extraction with dilute HCl,the extracts of swine liver homogenization was loaded on the IAC column for purification and determined by GC/MS.The limit of detection for salbutamol and clenbuterol were 0.5 ng/g and 0.8 ng/g,and the limit of quantification for salbutamol and clenbuterol were 2.0 ng/g and 2.5 ng/g respectively.Recoveries ranged 78.4%-106.9% for salbutamol and 77.1%-102.9% for clenbuterol at fortified levels of 1,5,10,20 ng/g in blank swine liver.It was the first time that the IAC-GC/MS for detection salbutamol and clenbuterol in swine liver was reported in China. |