| 标题： | ‘富有’和‘次郎’甜柿叶片再生植株的研究 |
|---|---|
| 英文标题： | Shoot Regeneration from Leaves of‘Fuyu’and‘Jiro’Persimmon |
| 作者： | 马俊莲,刘恺,张子德 |
| 英文作者： | Ma Junlian~1,Liu Kai~2,and Zhang Zide~1 ( ~1College of Food Science and Technology,Agricultural University of Hebei,Baoding,Hebei 071001,China,~2Department of Chemistry,Baoding Teachers College,Baoding,Hebei 071051,China) |
| 出版时间： | 2006-10-30 |
| 机构： | 河北农业大学食品科技学院,保定师范专科学校化学系,河北农业大学食品科技学院 河北保定071001,河北保定071051,河北保定071001 |
| 关键词： | 柿,TDZ,叶片,植株再生,愈伤组织 |
| 英文关键词： | Persimmon,TDZ,Leaf,Plant regeneration,Callus |
| 刊名： | 园艺学报 |
| 英文刊名： | 园艺学报 |
| ISSN： | 0513-353X |
| 期号： | 05 |
| 国内刊号： | 11-1924/S |
| 基金： | 河北省重点科技攻关项目(04395501D-3) |
| 主题： | ‘富有’和‘次郎’甜柿叶片再生植株的研究 |
| 页码： | 115-117 |
| 分类号： | S665.2； |
| 出版单位： | 园艺学报 |
| 数据来源： | CNKI |
| 是否SCI： | false |
| 是否EI： | false |
| 是否核心： | true |
| 摘要： | 比较了不同生长调节剂及其种类浓度和培养时间对‘富有’和‘次郎’两个甜柿(DiospyroskakiThunb.)品种的叶片愈伤组织诱导和不定芽再生的影响。TDZ诱导叶片愈伤组织和不定芽再生的能力很强,在含ZT0·5mg·L-1的培养基中,愈伤组织的形成率达96%,愈伤组织不定芽分化率达80%以上,由0·5mg·L-1TDZ诱导形成的富有和次郎愈伤组织,在含有ZT0·5mg·L-1的1/2DKW培养基培养30d,不定芽分化率均达96%以上,平均芽数分别为7·8和5·4,所得再生苗的生根率近75%。 |
| 英文摘要： | Studies were carried out on high-frequency shoot regeneration from leaves of Fuyu and Jiro,two Japanese non-astringent persimmon. The effects of growth regulator and cultural time on the adventitious shoot regeneration from leaves were investigated. The results showed that TDZ was a strong and efficient hormone inducing adventitious shoot and calli formation. The best regeneration was achieved in 1/2DKW+TDZ 0.5 mg·L~ -1 +ZT 1.0 mg·L~ -1 , in which callus percentage was 96% and shooting was near above 80%. After 30 day culture, the calli with small shooting were transferred to 1/2DKW medium supplemented with 0.5 mg·L~ -1 zeatin and 0.1 mg·L~ -1 IAA. Shooting regeneration rate were more than 96%, and average adventitious shoots per explant were 7.8 and 5.4 respectively for Fuyu and Jiro after 30 days culture. Rooting percentage from regenerated shoots was about 75%. |