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‘富有’和‘次郎’甜柿叶片再生植株的研究

标题: ‘富有’和‘次郎’甜柿叶片再生植株的研究
英文标题: Shoot Regeneration from Leaves of‘Fuyu’and‘Jiro’Persimmon
作者: 马俊莲,刘恺,张子德
英文作者: Ma Junlian~1,Liu Kai~2,and Zhang Zide~1 ( ~1College of Food Science and Technology,Agricultural University of Hebei,Baoding,Hebei 071001,China,~2Department of Chemistry,Baoding Teachers College,Baoding,Hebei 071051,China)
出版时间: 2006-10-30
机构: 河北农业大学食品科技学院,保定师范专科学校化学系,河北农业大学食品科技学院 河北保定071001,河北保定071051,河北保定071001
关键词: 柿,TDZ,叶片,植株再生,愈伤组织
英文关键词: Persimmon,TDZ,Leaf,Plant regeneration,Callus
刊名: 园艺学报
英文刊名: 园艺学报
ISSN: 0513-353X
期号: 05
国内刊号: 11-1924/S
基金: 河北省重点科技攻关项目(04395501D-3)
主题: ‘富有’和‘次郎’甜柿叶片再生植株的研究
页码: 115-117
分类号: S665.2;
出版单位: 园艺学报
数据来源: CNKI
是否SCI: false
是否EI: false
是否核心: true
摘要: 比较了不同生长调节剂及其种类浓度和培养时间对‘富有’和‘次郎’两个甜柿(DiospyroskakiThunb.)品种的叶片愈伤组织诱导和不定芽再生的影响。TDZ诱导叶片愈伤组织和不定芽再生的能力很强,在含ZT0·5mg·L-1的培养基中,愈伤组织的形成率达96%,愈伤组织不定芽分化率达80%以上,由0·5mg·L-1TDZ诱导形成的富有和次郎愈伤组织,在含有ZT0·5mg·L-1的1/2DKW培养基培养30d,不定芽分化率均达96%以上,平均芽数分别为7·8和5·4,所得再生苗的生根率近75%。
英文摘要: Studies were carried out on high-frequency shoot regeneration from leaves of Fuyu and Jiro,two Japanese non-astringent persimmon. The effects of growth regulator and cultural time on the adventitious shoot regeneration from leaves were investigated. The results showed that TDZ was a strong and efficient hormone inducing adventitious shoot and calli formation. The best regeneration was achieved in 1/2DKW+TDZ 0.5 mg·L~ -1 +ZT 1.0 mg·L~ -1 , in which callus percentage was 96% and shooting was near above 80%. After 30 day culture, the calli with small shooting were transferred to 1/2DKW medium supplemented with 0.5 mg·L~ -1 zeatin and 0.1 mg·L~ -1 IAA. Shooting regeneration rate were more than 96%, and average adventitious shoots per explant were 7.8 and 5.4 respectively for Fuyu and Jiro after 30 days culture. Rooting percentage from regenerated shoots was about 75%.