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治疗奶牛乳房炎中药透皮剂的研制与临床药效研究

标题: 治疗奶牛乳房炎中药透皮剂的研制与临床药效研究
英文标题: The Development and Studies on Pharmacodynamics Action of Transdermal Compound Traditional Chinese Medicine Agent on Clinical Mastitis of Dairy Cattle
作者: 张少华
出版时间: 2010-01-01
所在大学: 河北农业大学
关键词: 奶牛,乳房炎,中药,透皮剂,临床药效
英文关键词: dairy cattle,mastitis,traditional chinese medicine,transdermal agent,Clinical efficacy
论文级别: 硕士
学位: 学位论文
导师: 史万玉
专业: 临床兽医学
提交时间: 2010
摘要: 本试验采用琼脂平板扩散法(打孔法),研究了黄芩等21种中药对从乳房炎奶牛乳汁中分离出的3种主要致病菌的体外抑菌情况.结果发现,大肠杆菌对黄芩高度敏感,对丹参、青翘属中度敏感;金黄色葡萄球菌对黄芩、青翘、丹参、地榆、红花、蒲公英高度敏感,对枳壳、忍冬藤、萹蓄、苦地丁、蟾酥、木通中度敏感;无乳链球对地榆、当归属高度敏感,对青翘、黄芩、蒲公英、丹参、苦地丁、红花、川芎、枳壳中度敏感.为防治奶牛乳房炎有效中药复方的筛选提供了理论依据.本试验以硬脂酸甘油酯、凡士林等为基质,通过离心试验、耐寒耐热试验与加速试验,筛选最佳配比.试验结果表明,以硬脂酸甘油酯70g,硬脂酸100g,白凡士林120g,液体石蜡100g,甘油120g,三乙醇胺15g,山梨酸钾1g,蒸馏水424ml,中药50g,为最佳配比.该组方在离心试验、耐寒耐热试验与加速试验中均未见乳膏出现分层破乳、霉败以及色泽、均匀性改变等现象.乳膏剂应色泽均匀,质地细腻,有适当的黏稠性,易于涂布皮肤,无粗糙感,具有一定的相对稳定性.在温度3740℃,相对湿度75%以上的条件下可保存3个月.为了评价中药复方软膏剂透皮效果,本试验以中药软膏剂中绿原酸成分为标记物,运用高效液相色谱法(HPLC)测定透过液中绿原酸的含量,评价不同的中药剂型和不同的透皮促渗剂对中药软膏剂透皮效果的影响.试验结果中药超微粉直接入药的中药透皮剂中绿原酸的含量为174μg/g,超微粉煎剂入药的中药透皮剂中绿原酸的含量为88μg/g,普通煎剂入药的中药透皮剂中绿原酸的含量为62.1μg/g;含9%冰片为透皮剂的软膏剂其10h绿原酸透过量为5.94μg,含4.5%冰片+4.5%氮酮为透皮剂的软膏剂其10h绿原酸透过量为6.6μg,含9%氮酮为透皮剂的软膏剂其10h绿原酸透过量为4.02μg.试验结果表明:中药超微粉直接入药并以含4.5%冰片+4.5%氮酮作为促渗剂效果最佳.本试验通过皮肤刺激性试验检测中药透皮剂的安全性,试验结果表明皮肤有破损时积分均值为0.5,有轻微刺激性.皮肤完整时积分均值为0,无刺激性.临床药效研究表明中药透皮剂对奶牛乳房炎的治疗效果明显,可以有效地增强SOD、GSH-Px自由基清除酶活性,降低NO、MDA等自由基含量,达到治疗临床乳腺炎的目的.
英文摘要: Three kinds of major pathogenic microorganisms that cause mastitis of diary cows were selected and the in vitro antibiosis effects of 21 kinds of Chinese medicinal herbs such as Radix Scutellariae et.al. were tested through the method of stiletto. Twenty four nutrient agar medium were divided into there groups. Smear Escherichia Coli on A1-A8 nutrient agar medium homogeneous. Smear S. aurous on B1-B8 nutrient agar medium homogeneous. Smear Streptococcus agalactiae on C1-C8 nutrient agar medium homogeneous. Then slotting uniform upon the nutrient agar medium, respectively, each plate six hole. Add 20μL of every medical solution into each hole, and two holes were made to parallel samples. Add 21 chinese medicine to A1-A7 , B1-B7 , C1-C7, respectively,and A8,B8,C8 were the normal control which were added deionized water only. These nutrient agar medium were incubated at 37℃for 24 h. Bacterial existence and antibacterial circle diameter were observed. We found that different medicine for different strains produce different effects.The experiment results showed above supplied the scientific evidence for filtering Chinese medicinal herbs to prevent and cure the mastitis. This study evaluated the effects of Chinese herbal compound transdermal ointment, screened the best formula. With chlorogenic acid as marker, used high performance liquid chromatography (HPLC) determinated the content of chlorogenic acid in permeate liquid, evaluated different Chinese medicine formulations and different traditional Chinese medicine transdermal penetration enhancers on the traditional Chinese medicine Ointment transdermal effect. The result inditated that traditional Chinese medicine ultrafine powder directly to medicine and 4.5%borneol +4.5%Azone as penetration enhancers was best. The transdermal compound TCM agent stability test results showed there was no layered, emulsion-breaking, mildew, as well as change of color or uniformity in the centrifuge test, cold and heat test and Accelerated test. The transdermal compound TCM agent has a good stability, the stability of the results were satisfactory. A two-year period required for the product, at a temperature 37-40℃, relative humidity above 75%under the conditions to save 3 months, product stability, and can be tentatively valid for 2 years. Therefore, the shelf life of drug may be set temporarily for 2 years.The results of Clinieal pharmacodynamics experiment showed that the transdermal agent had good therapeutic action on clinical mastitis of dairy cattle curing. The studies showed Transdermal compound traditional Chinese medicine agent can effeetually strengthen activity of SOD,GSH-Px,degrade concentration of free radical NO,MDA in sera and whey from cows with clinical mastitis,to achieve objective cured bovine mastitis by Transdermal compound traditional Chinese medicine agent