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亚精胺对切花菊'神马'花芽分化期间内源多胺和激素的影响

标题: 亚精胺对切花菊'神马'花芽分化期间内源多胺和激素的影响
英文标题: Effects of Spermidine on the Contents of Endogenous Polyamines and Hormones during Flower Bud Differentiation of Cutting Chrysanthemum 'Jinba'
作者: 杨丽
出版时间: 2009-01-01
所在大学: 河北农业大学
关键词: 菊花,花芽分化,多胺,激素
英文关键词: Chrysanthemum morifolium,flower bud differentiation,polyamine,hormone
论文级别: 硕士
学位: 学位论文
导师: 杨际双
专业: 园林植物与观赏园艺
提交时间: 2009
摘要: 秋菊-切花菊'神马'(Chrysanthemum morifolium 'Jinba')是我国主要的出口切花品种,花期调控是其周年生产的关键.但是现在花期调控的研究比较少.近几年发现多胺(polyamines, Pas)与花芽分化有密切关系.本试验通过研究花芽分化期间顶芽和叶片中内源Pas和激素含量的变化以及不同浓度的亚精胺(spermidine, Spd)处理(0-1.0 mmol/L)对花芽分化期间内源Pas和激素以及花期的影响,研究了Pas和激素在'神马'花芽分化不同时期所起的调控作用,确定促进'神马'开花的最佳Spd溶液浓度,为Pas和激素对'神马'花芽分化的调节提出更全面的理论依据,以期达到对菊花进行花期调控的目的. 主要结果如下: 1.'神马'花芽分化分为9个时期,历时24 d结束.花芽分化开始后腐胺(putrescine, Put)急剧下降,此后一直保持较低的水平;顶芽中精胺(spermine, Spm)与 Spd含量呈平行波动上升趋势,Spd和Spm与总苞鳞片和小花原基分化有密切关系,顶芽中Spm处于优势地位,且顶芽和叶片的变化趋势相反,可能Spm从叶片向顶芽中转移. 激素的结果表明,花芽分化开始后赤霉素(gibberellic acid, GA3)先升高随后下降并保持低水平状态,花芽分化前期吲哚乙酸(Indole acetic acid,IAA),玉米素核苷(zeatin riboside, ZR)、异戊烯基腺苷(isopentenyl adenosine, iPA),脱落酸(abscisic acid, ABA)的含量较高,后期水平较低.花芽分化期间ABA/GA3、CTK/GA3 比值较高,而 IAA/CTK、ABA/IAA变化不明显. 2.0.1 mmol/L Spd处理提高了花芽诱导前期Spd和Spm的含量,降低了顶芽前期GA3的含量,提高了ABA的含量和ABA/GA3与CTK/GA3的比值,虽然提高了IAA的含量,但仍低于0.01、1 mmol/L处理的含量.1 mmol/L处理提高了Put和GA3的含量,并且抑制CTK的合成.0.1 mmol/L Spd处理效果最好,延长观赏天数,提高切花质量,1 mmol/L Spd处理抑制开花,缩短观赏天数.此外还发现随着Spd浓度的提高舌状花数也增多,因此Spd与小花原基的分化有密切关系. 结论:Put与GA3抑制花芽分化;Spd和Spm与总苞鳞片分化和小花原基分化有密切关系;Spd促进小花原基数目的增多;高浓度与低浓度的IAA抑制其花芽分化;CTK促进花芽分化;高含量的ABA诱导花芽分化,低含量的ABA促进花发育.
英文摘要: Autumn-cutting Chrysanthemum morifolium''''Jinba''''is the most important cutting flower for exporting. To produce them all year round, it is important to control the florescence. But the study on florescence regulation was small.These years we found that polyamines (PAs) and hormones were related to the bud differentiation. In this trial we studied the changes of PAs and hormones in the apicals and leaves and how the different concentrations of spermidine (Spd) (0, 0.01, 0.1, 1.0 mmol/L) affected the endogenous PAs and hormones, as well as the florescence. Then we could know which role did the PAs and hormones played, and make sure what the best concentrations to promote florescence were. It would be benefical to bring forward the theory of controlling florescence with PAs and hormones.The main results were as follows:1. The result showed that bud differentiation included 9 stages with 24 days. After differentiation, the contents of putrescine (Put) decreased significantly and then remained low in apical buds and leaves at other stages. Both spermine (Spm) and Spd in the apical buds increased at different stages. The Spd and Spm in apical buds and leaves could be related to the involucre primordia differentiation and floret primordium differentiation. In apicals buds, Spm predominated, and was different from the leaves, maybe transported from leaves to apical buds.The study of hormones showed that after differentiation, the contents of gibberellic acid (GA_3) decreased significantly and then remained low in apical buds and leaves at other stages. In the earlier stage indole acetic acid (IAA), zeatin riboside (ZR), isopentenyl adenosine (iPA) and abscisic acid (ABA) were higher; they decreased later. During the flower bud differentiation the ratio of ABA/ GA_3 and that of CTK/GA_3 were higher than that at vegetative stage, but the ratioes of IAA/CTK and ABA/IAA changed little.2. The contents of Spd, Spm and ABA were promoted at early stage, so as the ratio of ABA/GA_3 and CTK/GA_3 with the treatment of 0.1 mmol/L. But the contents of GA_3 decreased. Although the contents of IAA increased, it was lower than that of the other two treatments. The contents of Put and GA_3 were increased at early stages with the treatment of 1 mmol/L, and inhibited the CTK. In addition, the higher was the concentration, the more were the number of ray florets.In conclusion, Put and GA_3 inhibited floral bud differentiaion, but CTK promoted it. The Spd in apical buds and leaves could be related to floret primordium differentiation. Higher concentration of IAA inhibited it, and lower one promoted it. High contents of ABA could induce the floral bud differentiation, and low ones promote it development.