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日粮中添加共轭亚油酸对小鼠血清瘦素水平及卵泡发育的影响

标题: 日粮中添加共轭亚油酸对小鼠血清瘦素水平及卵泡发育的影响
英文标题: Effects of dietary conjugated linoleic acid on serum leptin and follicle development in mice
作者: 崔亚利,李三吓,李同洲,李敏,陈宝江
英文作者: CUI Ya-li,LI San-xia,LI Tong-zhou,LI Min,CHEN Bao-jiang
出版时间: 2015-01-01
机构: 河北农业大学,河北农业大学
关键词: 共轭亚油酸,瘦素,卵巢,小鼠
英文关键词: conjugated linoleic acid,leptin,ovary,mice
刊名: 河北农业大学学报
英文刊名: Journal of Agricultural University of Hebei
卷号: 3
基金: 河北省教育厅
页码: 5
摘要: "为了研究共轭亚油酸对小鼠卵泡发育的影响。将60只6~7周龄的K M 雌性小鼠,随机分为2组。对照组饲喂添加了1.5%花生油的繁殖颗粒料,试验组饲喂添加了1.5%花生油和1.5%共轭亚油酸的繁殖颗粒料。酶联免疫法测定不同生殖阶段血清瘦素浓度,常规石蜡切片法制备卵巢切片,观察卵巢的组织学结构并计数初级和次级卵泡数量。结果表明:(1)共轭亚油酸极显著降低了间情期、发情期、妊娠5 d小鼠的血清瘦素浓度(P<0.01),显著降低了妊娠9 d的血清瘦素浓度(P<0.05)。(2)共轭亚油酸对初级卵泡数量没有显著影响,极显著增加发情期次级卵泡的数量和产仔数。由此可见,共轭亚油酸可能通过降低血清瘦素水平,增加发情期次级卵泡数量,进而增加排卵数影响小鼠的生殖功能。"
英文摘要: "This study was aimed at disclosing the effects of conjugated linoleic acid on follicle development of mice .Sixty female mice ,6 ~ 7 week‐old ,were randomly divided into two groups on the basis of the diet to be fed as follows :1 .5% peanut oil (control group);1 .5%peanut oil and 1 .5% conjugated linoleic acid diet (experimental group) .Serum leptin was de‐termined by ELISA .Paraffin section of ovaries was made to calculate primary and secondary follicles .The results showed as the follows :(1)Serum leptin in dioestrous ,oestrous ,5 days of pregnancy and 9 days of pregnancy were significantly lower in conjugated linoleic acid‐fed group than that of in peanut oil merely diet group .(2) No change of the numbers of primary follicles by conjugated linoleic acid was observed .Dietary conjugated linoleic acid increased the numbers of secondary follicles (P<0 .01) in ovary of oestrous .It was concluded that dietary conjugated linoleic acid affected reproductive function of mice by decreasing serum leptin ,in‐creasing the numbers of secondary follicles ,promoting the number of ovulation ."