标题: |
磷脂酶C特异性抑制剂U-73122对玉米大斑病菌分生孢子萌发及毒素活性的影响 |
英文标题: |
Effects of Phospholipase C Inhibitor U-73122 on Conidial Germination and HT-toxin Activity of Setosphaeria turcica |
作者: |
郝志敏,郝杰,李志勇,董金皋 |
英文作者: |
HAO Zhi-min,HAO Jie,LI Zhi-yong,DONG Jin-gao |
出版时间: |
2010-01-01 |
机构: |
河北农业大学 |
关键词: |
玉米大斑病菌,磷脂酶C,U-73122,分生孢子,毒素 |
英文关键词: |
Setosphaeria turcica,Phospholipase C,U-73122,Conidia,HT-toxin |
刊名: |
华北农学报 |
英文刊名: |
ACTA AGRICULTURAE BOREALI-SINICA |
ISSN: |
1000-7091 |
期号: |
25 |
卷号: |
006 |
基金: |
^A河北省自然科学基金^BC2008000335^D1 |
页码: |
118-121 |
分类号: |
S432.44 |
摘要: |
磷脂酶C(Phospholipase C,PLC)通过降解细胞膜上的磷脂分子产生IP,和DAG两个第二信使分子,分别激活下游的Ca2+途径和蛋白激酶C.研究表明,PLC与植物病原真菌的致病及发育过程有关.利用PLC特异性抑制剂U-73122初步研究了玉米大斑病菌PLC的功能.结果显示,U-73122对玉米大斑病菌菌丝生长及分生孢子的萌发均具有明显的抑制作用,且抑制率随抑制剂浓度升高而增强;并且可引起芽管的缩短和不规则膨大.此外,U-73122也可以导致病菌粗毒素的生物学活性明显降低.由此说明PLC可以通过影响菌丝及芽管的极性生长,而调控玉米大斑病菌的菌丝及分生孢子发育;同时参与病菌的毒素合成. |
英文摘要: |
Phospholipase C(PLC)could catalyze the hydrolysis of phospholipid molecules on cell membrane and generate two second messenger molecules IP3 and DAG,which activated Ca2+ signal pathway and protein kinase C respectively.It had been proved that PLC modulated pathogenicity and development of plant pathogenic fungi.In this research,U-73122,the specific inhibitor of PLCs,was used to study functions of PLCs in Setosphaeria turcica preliminary.U-73122 showed significant inhibition effects on hyphal growth and coni... |