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苹果砧木幼苗根系分泌物的分离与鉴定

标题: 苹果砧木幼苗根系分泌物的分离与鉴定
英文标题: ISOLATION AND IDENTIFICATION OF ROOT EXUDATES IN DIFFERENT KINDS OF APPLE ROOTSTOCK SEEDLINGS
作者: 张江红,张殿生,毛志泉,李中勇,赵明新
英文作者: ZHANG JIANG-HONG,ZHANG DIAN-SHENG,MAO ZHI-QUAN,LI ZHONG-YONG,ZHAO MING-XIN
出版时间: 2009-01-01
机构: 河北农业大学
关键词: 苹果砧木,根系分泌物,HPLC,酚类物质
英文关键词: APPLE ROOTSTOCK,ROOT EXUDATE,HPLC,PHENOLICS
刊名: 河北农业大学学报
英文刊名: JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI
ISSN: 1000-1573
卷号: 004
基金: ^A引进国际先进农业科学技术计划(“948”计划)^B2006-G28^D1%^A农业部行业科技项目^BNYHYZX07-024^D2%^A国家苹果产业技术体系建设专项经费资助项目^B^D3
页码: 29-32
分类号: S661.1
摘要: 以4种苹果砧木幼苗为试材,利用高效液相色谱法(HPLC)测定根系分泌酚类物质的含量,并比较不同砧木品种间根系分泌物成分的差异.结果表明:采用C18分离柱,波长280 NM,流动相为乙腈和水进行梯度洗脱,流速1 ML/MIN,分离效果良好.在一定浓度范围内,10种酚类物质的峰面积与浓度间存在显著的线性关系.不同苹果砧木根系分泌物种类和数量不同,主要成分为间苯三酚、根皮素、根皮苷和苯甲酸,分泌物含量从高到低顺序依次为''怀来海棠''、''新疆海棠''、''莱芜难咽''、''平邑甜茶''.
英文摘要: The main phenolic compounds in root exudates of apple rootstock seedlings were identified and qualified using high-performance liquid chromatography (HPLC). The results showed that the optimal chromatographic separation was achieved on a reverse phase C18 column with acetonitrile-water as the mobile phase using gradient elution, at 280 nm, with the flow rate of 1 mL/min. The peak area was linear with the phenolic concentration in the range of 0.01-1.9 μg/g. The main phenolic compounds were phloroglucinol, phloretin, phloridzin, and benzoic acid. The contents of detected phenolic compounds, in a descending order, in root exudates of apple rootstocks was as follows: huailaihaitang (Malus micromalus Makino.)> xinjianghaitang (Malus sieversii Roem)>laiwunanyan (Malus micromalus M) > pingyitiancha (Malus hepenhensis Rehd.).