||30 milk samples of clinical dairy cow mastitis were collected from a dairy cow farm of Tangshan area, and the isolation, culture and biochemical identification of pathogenic bacteria, and drug susceptibility test were carried out in this study. The results showed that 156 bacteria and 8 species were isolated from30 samples, including 58 Staphylococ-cus aureus (37.2%), 17 Escherichia Coli (10.9%), 10 Staphylococcus epidermidis 6.4%),38 Streptococcus uberis(24.4%), 15 Streptococcus mastitidis(9.6%), 16 Streptococcus dysgalactiae (10.3%), 1 Klebsiella(0.6%), 1 Bacillus termo (0.6%). Staphylococci and Streptococcus were the main bacteria for causing cow clinical mastitis. The second pathogens were Streptococcus, accounted for 98.7% (154/156). The drug susceptibility test showed that the most sensitive antibiotics to pathogenic bacteria were TCM formula l(dandelion, fructus forsythiae, Snakegourd Fruit, Ricepaperplant Pith, Szechuan Lovage Rhizome, Rhizoma Bolbostemmae, cotton rose hibiscus leaf, honeysuckle, Cowherb Seed, Chinese Angelica), TCM formula 2(Raidx Astragali, Codonopsis pilosula, Chinese Angelica, Ricepaperplant Pith, Szechuan Lovage Rhizome, Largehead Atractylodes Rhizome, akebiaquinata, LiquoricRoot, Cowherb Seed, Beautiful Sweetgum Fruit), compound recipe of Huanglian, Herbahouttuyniae injection, Neomycin, sulfate.