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灰葡萄孢产孢缺陷菌株的遗传分析

标题: 灰葡萄孢产孢缺陷菌株的遗传分析
英文标题: Genetic Analysis of Sporulation Defective in Botrytis cinerea
作者: 张玉净,郝志敏,郑蒙,张金林,董金皋
英文作者: ZHANG Yu-jing,HAO Zhi-min,ZHENG Meng,ZHANG Jin-lin,DONG Jin-gao
出版时间: 2011-01-01
机构: 河北农业大学
关键词: 灰葡萄孢,产孢量,TAIL-PCR,RT-PCR
英文关键词: Botrytis cinerea,Conidiation,TAIL-PCR,RT-PCR
刊名: 华北农学报
英文刊名: ACTA AGRICULTURAE BOREALI-SINICA
ISSN: 1000-7091
期号: 26
卷号: 003
基金: ^A河北省自然科学基金^B08B021^D1
页码: 86-89
分类号: S436.631
摘要: 本研究从400余株灰葡萄孢ATMT转化子中筛选到产孢缺陷菌株BCG-183.利用CTAB法提取野生型和BCG-183的基因组DNA,Southern杂交证实T-DNA标记在突变菌株中为单拷贝插入;对该菌株表型进行分析,发现BCG-183菌株菌落呈白色,菌丝产量较野生型大;致病性较野生型强;利用血球计数板计算产孢量,表明BCG-183不产生分生孢子;胞壁降解酶活性比野生型强.利用TAIL-PCR技术确定T-DNA插入位点侧翼序列,生物信息学分析表明,T-DNA插入到灰葡萄孢基因组超级重叠群42(Supercontig 42)中假定蛋白编码基因(BClG_07455)的3'末端.RT-PCR结果表明,BCJG_07455基因在突变菌株中没有表达.推测BC1G_07455可能与灰葡萄孢产孢相关.
英文摘要: Conidiation-deficiency transformant BCG-183 was obstained from more than 400 ATMT transformants.The genomic DNA of wild type and BCG-183 were extracted by CTAB method,and southern blot showed that one copy of T-DNA had inserted into the mutant''s DNA.By analysing the phenotype of the mutant,we found that mutant''s hyphae were more dense than the wild type,while its colony was white.The mutant didn''t produce conidium.And the cell-wall-degrading enzyme activities were stronger than that of the wild type′s.T-DNA...