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灰葡萄孢菌核发育相关基因的功能分析

标题: 灰葡萄孢菌核发育相关基因的功能分析
英文标题: Functional Analysis of a Gene Related to Sclerotia Formation in Botrytis cinerea
作者: 司贺龙,赵斌,赵福鑫,邢继红,韩建民,董金皋
英文作者: SI He-long,ZHAO Bin,ZHAO Fu-xin,XING Ji-hong,HAN Jian-min,DONG Jin-gao
出版时间: 2014-01-01
机构: 河北农业大学
关键词: 灰葡萄孢,T-DNA插入突变体,TAIL-PCR
英文关键词: Botrytis cinerea,T-DNA insertional mutant,TAIL-PCR
刊名: 华北农学报
英文刊名: Acta Agriculturae Boreali-Sinica
ISSN: 1000-7091
卷号: 002
基金: ^A河北省科技计划项目^B(12226507)^D1%^A河北农业大学青年科学基金项目^B(QJ201235,QJ201231)^D2
页码: 80-84
分类号: S436.631
摘要: 从灰葡萄孢ATMT 突变体库中筛选获得一株不产生菌核的突变体,并利用TAIL-PCR技术获得插入位点的侧翼序列,将其在灰葡萄孢基因组数据库中进行Blast比对分析,采用分子生物学技术,对突变菌株BMH174鉴定并获得其突变基因(BC1G_06945.1);并对突变体的形态学、生长速率、分生孢子产量、致病能力、胞壁降解酶活力、毒素的生物学活性等方面进行了研究,这将为深入了解灰葡萄孢的致病机制奠定基础。
英文摘要: One sclerotia-defect mutant of Botrytis cinerea BMH174 was obtained by Agrobacterium tumefaciens-mediated method. The flanking sequence was acquired by TAIL-PCR,and then the T-DNA insertion gene confirmed by Blast in the B. cinerea gene database. The mutant gene was identified BC1G_06945. 1 gene by molecular biolo-gy. The sclerotia formation and pathogenicity in B. cinerea which will facilitate to understand molecular mechanism of conidium development,sclerotia formation and pathogenic in B. cinerea.