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大白菜子叶高频再生体系的建立

标题: 大白菜子叶高频再生体系的建立
英文标题: ESTABLISHMENT OF HIGH FREQUENCY REGENERATION SYSTEM FROM COTYLEDON IN CHINESE CABBAGE
作者: 马茜,陈雪平,罗双霞,申书兴,王彦华
英文作者: MA QIAN,CHEN XUE-PIN,LUO SHUANG-XIA,SHEN SHU-XING,WANG YAN-HUA
出版时间: 2009-01-01
机构: 河北农业大学
关键词: 大白菜,子叶,再生频率,TDZ,2-4-D
英文关键词: CHINESE CABBAGE,COTYLEDON,REGENERATION FREQUENCY,TDZ,2,4-D
刊名: 河北农业大学学报
英文刊名: JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI
ISSN: 1000-1573
卷号: 005
基金: ^A河北省自然科学基金^BC2006000416^D1%^A河北农业大学博士启动基金^B^D2
页码: 36-39
分类号: S634.1
摘要: 以大白菜(BRASSICA CAMPESTRIS L SSP. PEKINENSIS)为材料,MS为基本培养基,研究了不同基因型、不同外植体类型、不同激素组合及预培养处理条件对不定芽再生的影响.结果表明:基因型、外植体类型、激素组合和预培养条件对不定芽的形成均具有十分明显的影响.3个基因型间,''85-1''的再生频率最高,''天津青麻叶''次之,''北京新三号''最低;2种外植体中,带柄子叶的再生频率显著高于下胚轴;22个激素组合中, TDZ 0.7 MG/L + NAA 0.5 MG/L组合的再生频率最高(72.5%,''85-1''带柄子叶);7个预培养处理中,0.5 MG/L 2,4-D预培养1 D的效果最好,不定芽再生频率达到87.7%,且芽数多、质量好.
英文摘要: Cotyledon with petiole and hypocotyl of three different varieties of Chinese cabbage as explants,the effects of hormone combination of thidiazuron (TDZ) + naphthaleneacetic acid (NAA) and benzyladenine (BA) + NAA on adventitous bud regeneration were studied. The results showed that the difference of the regeneration frequency among the difference varieties were significant, and that the regeneration frequency of cotyledon with petioles were better than hypocotyl, in which the variety ''85-1'' had the highest frequency, followed by ''Tianjin Qinmaye'',and ''Beijing Xinsanhao'' had the lowest. The effect of reducing adventitous buds of TDZ was better than that of 6-BA. The frequency could reach 72. 5%in the MS medium containing TDZ 0. 7 mg/L+NAA 0. 5 mg/L,and if the explants were cultured 1d in the MS medium with 2,4-D 0. 5 mg/L before being transferred to the medium with TDZ 0. 7mg/L + NAA0. 5 mg/L, the frequency could reach as high as 87. 7%. The establishment of high frequency regeneration system of Chinese cabbage laid a foundation for the genetic transformation.