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樟子松成熟胚的离体培养与不定芽的形成

标题: 樟子松成熟胚的离体培养与不定芽的形成
英文标题: IN VITRO FORMATION OF ADVENTITIOUS BUDS FROM EMBRYOS OF PINUS SYLVESTRIS VAR. MONGOLICA
作者: 康明,田建强,郑均宝
英文作者: KANG MING,TIAN JIANQIANG,ZHENG JUNBAO
出版时间: 1993-01-01
机构: 河北农业大学
关键词: 胚培养,不定芽,分化,樟子松
英文关键词: IN VITRO CULTURE OF EMBRYO,ADVENTITIOUS BUD,DIFFERENTIATION,PINUS SYLVESTRIS VAR MONGOLICA.
刊名: 河北林学院学报
ISSN: 1007-4961
卷号: 004
页码: 273-277
摘要: 沙藏45d的樟子松种子,剥胚培养在5个系列17种培养基上.在6BA0.2~2.0(mg/L,单位下同)+NAA0.05~0.5以及6BA1.0+IAA0.2~0.5的激素配比范围内,可诱导胚形成不定芽.KT与NAA或IAA配合时无不定芽形成.不定芽分化部位明显受激素种类控制,6BA与NAA配合可诱导胚轴分化出不定芽,而6BA与IAA配合,则可从子叶顶端和基部诱导出不定芽.
英文摘要: This paper reports the regeneration of adventitious buds from mature embryos of P. sylvestris var mongolica. Embryos, excised ascpilically from seeds which were treated by stratification for 45 days, were cultured on 17 kinds of media. The adventitious buds arose from cxptant when growth regulators of 6BA 0.2~2.0mg/L +NAA 0.05~0.5mg/ L or 6BA 1.0mg/ L +IAA 0.2-0.5 mg/ L were supplemented to media. Noncbuds differentiated from embryos when KT was substituted for 6BA. The locations and patterns of adventitiou...