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转基因杨树中外源Bt基因mRNA及其蛋白运输

标题: 转基因杨树中外源Bt基因mRNA及其蛋白运输
英文标题: Transportation of the mRNA and Protein of the Foreign Bt Gene in Transgenic Poplar
作者: 王连荣,杨敏生
出版时间: 2010-01-01
机构: 河北农业大学
关键词: mRNA运输,Bt毒蛋白运输,嫁接,抗虫性
英文关键词: transportation of mRNA,transportation of Bt toxin protein,grafting,insect-resistance
刊名: 林业科学
英文刊名: Scientia Silvae Sinicae
ISSN: 1001-7488
卷号: 007
基金: ^A国家自然科学基金^B30872050^D1%^A河北省自然科学基金^BC2006000464^D2%^A转基因生物新品种培育重大专项^B2009ZX08011-027B^D3
页码: 49-54
分类号: S792.11
摘要: 以741杨和转BtcrylAc基因741杨抗虫株系Pb29互为接穗和砧木进行嫁接,利用RT-PCR和ELSA技术对Bt基因的mRNA及其蛋白是否在砧木与接穗间运输进行研究.RT-PCR检测结果表明:以741杨为接穗或砧木的嫩枝和嫩叶中均未检测到Bt基因的mRNA,说明m基因的mRNA没有在砧木与接穗间进行运输.ELISA检测发现,各嫁接处理的741杨砧木和接穗的叶片、韧皮部、木质部和髓中均检测出Bt毒蛋白的存在,证明Bt毒蛋白可以通过嫁接的方式在砧木和接穗间进行运输.用各嫁接处理接穗叶片在室内喂饲杨扇舟蛾幼虫,发现嫁接转基因杨树的非转基因杨可提高对杨扇舟蛾幼虫的致死率,延长发育历期,表现出一定的抗虫性.
英文摘要: In this paper poplar741 and Pb29 transgenic poplar741with Btcry1Ac gene were grafted as scion and stock mutually,to investigate whether mRNA and protein of Bt gene would be transported between the stock and scion by means of RT-PCR and ELISA techniques. The result of RT-PCR test showed that mRNA of Bt gene was not detected in the branch and leaf of poplar741 no matter its material was used as scion or stock,which suggested that mRNA of Bt gene was not transported between the stock and scion. The result of ELISA test showed that Bt toxin protein was detected in the leaf,phloem,xylem and pith of poplar741 in the grafted plants,which demonstrated that Bt toxin protein was transported between the stock and scion in the grafted plants. To find out any influence of grafting on larvae of Clostera anachoreta, the larvae were fed indoors on the leaves of grafted scion. The results showed that grafting the poplar741 to transgenic ones could increase the death rate,extend the larval developmental period of C. anachoreta,and express certain resistance to insects.