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瘦素及其长型瘦素受体在雌性小鼠体内的表达

标题: 瘦素及其长型瘦素受体在雌性小鼠体内的表达
英文标题: EXPRESSION OF LEPTIN AND LONG-LEPTIN RECEPTOR IN THE FEMALE MOUSE
作者: 邓艳幔,崔亚利,胡满,王宝
英文作者: DEND YAN-MAN,CUI YA-LI,HU MAN,WANG BAO
出版时间: 2010-01-01
机构: 河北农业大学
关键词: 瘦素,瘦素受体,免疫组织化学,ELISA,小鼠
英文关键词: LEPTIN,LEPTIN RECEPTOR,IMMUNOHISTOCHEMISTRY,ELISA,MOUSE
刊名: 中国兽医学报
英文刊名: CHINESE JOURNAL OF VETERINARY SCIENCE
ISSN: 1005-4545
卷号: 003
基金: ^A河北省教育厅资助项目^B2006119^D1
页码: 414-418
分类号: S865.13
摘要: 采用免疫组织化学SP染色法时长型瘦素受体在雌性小鼠生殖周期不同阶段的下丘脑、卵巢、子宫中细胞定位和分布进行了研究,并用ELISA方法时小鼠血清中的瘦素浓度进行了检测.结果显示,下丘脑神经元胞质中有棕褐色阳性颗粒,且阳性细胞数量随妊娠日龄增加逐渐增加,妊娠期与间情期阳性细胞数差异显著(P<0.05);在卵巢中,卵母细胞胞质中有长型瘦素受体表达,随卵泡的发育,卵泡细胞中免疫反应阳性细胞数量逐渐增加;在胚泡附植期,瘦素受体在子宫腺和子宫内膜上皮细胞中大量表达.妊娠期血清瘦素浓度均高于间情期,且瘦素浓度从间情期到妊娠4日龄,呈上升趋势,妊娠5日龄稍下降,后随妊娠日龄逐渐增加.结果表明,瘦素及其长型受体能够促进卵泡发育,有利于小鼠胚泡的附植.
英文摘要: We studied the expression of long-leptin receptor positive cells in the hypothalamus,ovarian,uterine at different stages of reproductive cycle in the female KM mouse by immunohistochemistry staining method.The concentration of serum leptin was tested by ELISA method.The results showed:there were many tan-positive particles in the neuron cytoplasm of hypothalamus,and the tan-positive cells increased with pregnant days.We found that there was significant difference between dioestrus and pregnancy (P