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球孢白僵菌不同分离株的生物学特性的研究

标题: 球孢白僵菌不同分离株的生物学特性的研究
英文标题: Study on Biological Characteristics of Different Beauveria bassiana Strains
作者: 魏灵燕,宋健,曹伟平,冯书亮,王金耀,朱宝成,杜立新
英文作者: WEI Lingyan1,2,SONG Jian2,CAO Weiping2,DU lixin2,WANG Jinyao2,ZHU Baocheng1,FENG Shuliang2(1.College of Life Sciences,Hebei Agriculture University,Baoding 071000,2.Institute of Plant Protection,Hebei Academy of Agriculture and Forestry Sciences/IPM Centre of Hebei Province,China)
出版时间: 2012-01-01
机构: 河北农业大学
关键词: 球孢白僵菌,培养特征,孢子萌发特征
英文关键词: Beauveria bassiana,cultivate characteristics,germinated spores characteristics
刊名: 中国生物防治学报
英文刊名: Chinese Journal of Biological Control
ISSN: 2095-039X
卷号: 004
基金: ^A国家高技术研究发展计划(863计划)^B^D1%^A引进国际先进农业科学技术计划(948计划)^B^D2%^A河北省财政项目F12C10034^B^D3
页码: 502-507
分类号: S476.12
摘要: 为了明确不同白僵菌菌株的生物学特征,对15株白僵菌菌株的ITS片段进行测序分析,并对这些菌株的菌落生长速率、产孢量、孢子萌发率、单芽管孢子萌发比例、单芽管平均长度和长芽管平均长度等多项生物学指标进行测定.结果表明,单芽管孢子萌发比例变化范围在11.91%~96.03%;单芽管平均长度和双芽管平均长度分别为6.28~21.43μm和10.338~19.902μm;各菌株间孢子萌发率差异很大,变化范围22.09%~86.32%.对菌株的培养特性(孢子萌发率、产孢量和菌落生长速率)与孢子萌发特性(单芽管孢子萌发比例、芽管总平均长度和芽管整齐度)这两个主因子进行典型相关分析,两性状集团总体相关不显著(P=0.341),而孢子萌发率与单芽管孢子萌发比例相关显著,菌落生长速率与芽管总平均长度呈显著相关.
英文摘要: Based on ITS nucleotides sequence,fifteen Beauveria strains were identified to be Beauveria bassiana. Morphology feature and culture characteristics showed that the ratio of single germ tube spores of these strains was 11.91%-96.03%.The average length of single germ tube and double germ tube were 6.28-21.43 μm and 10.338-19.902 μm,repectivelty.The spore germination rate was 22.09%-86.32%.No significant correlation was observed between the micro-germinated spores characteristics and macro-cultivate character...