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玉米大斑病菌原生质体遗传转化体系的建立

标题: 玉米大斑病菌原生质体遗传转化体系的建立
英文标题: Establishment of the Genetic Transformation System of Protoplasts from Setosphaeria turcica
作者: 王文秀,谷守芹,李坡,范钰,董金皋
英文作者: WANG Wen-xiu,GU Shou-qin,LI Po,FAN Yu,DONG Jin-gao
出版时间: 2011-01-01
机构: 河北农业大学
关键词: 玉米大斑病菌,原生质体,遗传转化
英文关键词: Setosphaeria turcica,Protoplast,Genetic transformation
刊名: 玉米科学
英文刊名: Journal of Maize Sciences
ISSN: 1005-0906
期号: 19
卷号: 004
基金: ^A国家自然科学基金^B30471126^D1%^A河北省自然科学基金^BC2009000622^D2
页码: 134-137
分类号: S435.131
摘要: 以玉米大斑病菌菌株01-23为出发菌株,对影响原生质体遗传转化的主要条件进行了研究,包括原生质体制备的材料、酶解系统、稳渗剂、载体浓度及线性化程度等.结果表明,改良Fries培养基中培养24 h的分生孢子悬浮液是原生质体制备的首选材料;2%溶壁酶和1%蜗牛酶两种酶混用是最佳的酶解系统;0.7 mol/L NaCl是最适的稳渗剂;环状载体比线性载体转化效率高5倍以上;在载体浓度为100 μg/mL时转化效率最高.
英文摘要: The Setosphaeria turcica strain 01-23 was used to study main conditions which had impact on proto-plast genetic transformation,including the materials of protoplast preparation,the enzyme system,the osmotic stabiliz-er,the concentration and linearized degree of vector and so on.The results indicated that the conidial suspension in-cubated for 24 h was the optimal material for the preparation of protoplasts.The mixture of 2%Lywallzyme and 1%Snailase was the best enzyme system.0.7 mol/L NaCl was the most ap...