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'Siberia'百合再生体系的建立

标题: 'Siberia'百合再生体系的建立
英文标题: Establishment of regeneration system of Lilium 'Siberia'
作者: 赵芳,杨利平
出版时间: 2013-01-01
机构: 河北农业大学
关键词: 东方百合,再生体系,组织培养,分化率,增值系数
英文关键词: oriental lily,regeneration system,tissue culture,differentiation rate,multiplication coefficient
刊名: 河北农业大学学报
英文刊名: Journal of Agricultural University of Hebei
ISSN: 1000-1573
卷号: 002
基金: ^A重庆市自然科学基金^BCSTC,2011BB1001^D1%^A重庆市涪陵区科技项目^BFLKJ,2012BB1086^D2
页码: 60-64
分类号: S682.29
摘要: 以东方百合‘Siberia’离体鳞片为外植体,研究不同激素质量浓度对芽的诱导、增殖、小鳞茎形成、叶片和叶柄再生及生根的影响。结果显示:最适不定芽诱导培养基为MS+6-BA 2.0mg/L+NAA 0.5mg/L,分化率85%;最适增殖培养基为MS+6-BA 0.7mg/L+NAA 0.2mg/L,芽增值系数为3.83;不定芽形成鳞茎的适宜培养基为:MS+6-BA 0.2mg/L+NAA 0.5mg/L+7%蔗糖,增值系数2.47。鳞片叶片的最适再生培养基为MS+6-BA 0.5mg/L+2,4-D 1.0mg/L,分化率为92.5%;叶柄的最佳再生培养基为MS+6-BA 1.0mg/L+NAA 0.5mg/L,分化率为83.3%;暗培养对鳞片叶片及叶柄的再生无显著性的差异,分化率均在80%以上;最适生根培养基为1/2MS+NAA 0.2mg/L,生根率100%。
英文摘要: The bulb scales of the oriental lily 'Siberia' were treated as initial explants to test the inducement of bud,the bulb formation,the regeneration of leaf and leafstalk and rooting under different hormone concertrations.The result indicated that the optimal medium for adventitious bud came to be MS+6-BA 2.0 mg/L+NAA 0.5 mg/L with the differentiation rate of 85%.The most appropriate culture medium for multiplication was MS+6-BA 0.7 mg/L+NAA 0.2 mg/L,and the multiplication coefficient of bud was 3.83.The best ...