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高速逆流色谱分离小孢拟盘多毛孢PM-1菌株中的除草活性物质

标题: 高速逆流色谱分离小孢拟盘多毛孢PM-1菌株中的除草活性物质
英文标题: Preparative separation of the herbicidal substance from Pestalotiopsis microspora PM-1 by high-speed counter-current chromatography
作者: 王珺瑫,郑超,张利辉,董金皋
出版时间: 2013-01-01
机构: 河北农业大学
关键词: 小孢拟盘多毛孢,代谢产物,高速逆流色谱,除草活性,分离
英文关键词: Pestalotiopsis microspora,Metabolites,High-speed counter-current chromatography,Herbicidal activity,Separation
刊名: 微生物学通报
英文刊名: Microbiology China
ISSN: 0253-2654
卷号: 004
基金: ^A国家高技术研究发展计划(863计划)^B2011AA10A206^D1
页码: 617-625
分类号: S482.4
摘要: 【目的】应用高速逆流色谱法(High-speed counter-current chromatography,HSCCC)实现对小孢拟盘多毛孢Pestalotiopsis microspora PM-1菌株代谢产物中除草活性物质的首次分离。【方法】以正己烷:乙酸乙酯:甲醇:水(4:5:4:5,V/V/V/V)为最佳的两相溶剂体系,上相(水相)为固定相,下相(有机相)为流动相,正相洗脱。【结果】在流速2 mL/min、转速900 r/min、检测波长254 nm的条件下进行分离,得到4个馏分,其中馏分Ⅱ对马唐的活性较强。馏分Ⅱ经高效液相色谱法(High performance liquid chromatography,HPLC)以乙腈:水=75:25(V/V)为流动相经过C18柱进一步分离检测,所得馏分A对马唐种子萌发有较强的抑制作用。其保留时间为7.954 min,该峰经二极管阵列光谱检测为单一组分。【结论】利用最佳的高速逆流色谱条件和高效液相色谱条件对小孢拟盘多毛孢Pestalotiopsismicrospora PM-1菌株代谢产物进行分离,可获得除草活性化合物——馏分A。
英文摘要: [Objective] The separation of a herbicidal active compound from Pestalotiopsis microspora PM-1 was to be accomplished by high-speed counter-current chromatography(HSCCC) for the first time.[Methods] The compound was separated by HSCCC from the ethyl acetate crude extract of Pestalotiopsis microspora PM-1 with the two-phase solvent system consisting of n-hexane:ethyl acetate:methanol:water:solution(4:5:4:5,V/V/V/V).The upper phase(aqueous phase) was chosen as the stationary phase and the lower phase(organic ...