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利用枣离体叶片直接再生完整植株

标题: 利用枣离体叶片直接再生完整植株
英文标题: DIRECT PLANT REGENERATION FROM IN VITRO LEAVES OF CHINESE JUJUBE
作者: 王娜,刘孟军,秦子禹
英文作者: WANG NA,LIU MENG-JUN,QIN ZI-YU
出版时间: 2010-01-01
机构: 河北农业大学,河北农业大学
关键词: 枣,叶片,离体,直接再生,不定芽
英文关键词: ZIZIPHUS JUJUBA,LEAF,IN VITRO,DIRECT REGENERATION,ADVENTITIOUS SHOOT
刊名: 园艺学报
英文刊名: ACTA HORTICULTURAE SINICA
ISSN: 0513-353X
卷号: 001
基金: ^A国家科技支撑计划项目^B2007BAD36B07;2008BAD92B03^D1%^A河北省自然科学基金^BC2007000452^D2
页码: 103-108
分类号: S665.1
摘要: 以冬枣组培苗叶片为材料进行离体再生培养,通过对再生条件的系统研究,实现了离体叶片直接再生不定芽并获得完整植株.试验结果表明:麦芽糖为离体叶片直接再生的适宜碳源;AGNO_3可显著提高叶片不定芽再生率和出芽数,在TDZ 1.0 MG·L~(-1)+AGNO_31.0 MG·L~(-1)的条件下,不定芽直接再生率高达97.3%,出芽数达14.4个.但麦芽糖不适宜继续作为不定芽伸长培养的碳源,再生的不定芽需在MS+蔗糖40 G·L~(-1)+琼脂4 G·L~(-1)+BA 1.0 MG·L~(-1)+IBA 0.5 MG·L~(-1)的培养基上进行增殖,增殖系数为3.2.在IBA1.0 MG·L~(-1)条件下,生根率达87.3%.
英文摘要: A system of complete plantlet regeneration directly from in vitro leaf of Chinese jujube (Ziz-iphus jujuba Mill. ''Dongzao'') was established after investgating proper culture conditions. Carbon source plays a key role on direct shoot inducement from in vitro leaf and maltose is the proper carbon source. AgNO_3 could significantly improve the shoot regeneration rate and increase the shoot numbers. Under the medium of (97.3%) and shoot numbers (14.4) were obtained. But maltose was not suitable for the subsequent shoot growth. Regenerated shoots were growed and proliferated on medium of MS + sucrose 40 g·L~(-1) + agar 4 g·L~(-1) + BA 1.0 mg ·L~(-1) + IBA 0.5 mg·L~(-1) with multiplication coefficient of 3.2. The complete plantlets were obtained after the shoots were rooted on MS medium plus IBA 1.0 mg·L~(-1) with rooting rate of 87.3%.