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CXCR2基因多态性与奶牛体细胞评分的关联性

标题: CXCR2基因多态性与奶牛体细胞评分的关联性
英文标题: ASSOCIATION WITH CXCR2 GENE POLYMORPHISM AND SCS IN DAIRY COW
作者: 刘文娇,孙少华,李雪梅,孙志颖,倪俊卿,马亚宾
英文作者: LIU WEN-JIAO,SUN SHAO-HUA,LI XUE-MEI,SUN ZHI-YING,NI JUN-QING,MA YA-BIN
出版时间: 2010-01-01
机构: 河北农业大学,河北农业大学
关键词: 奶牛,乳房炎,体细胞评分,CXCR2基因,多态性
英文关键词: DAIRY COW,MASTITIS,SCS,CXCR2 GENE,POLYMORPHISM
刊名: 中国兽医学报
英文刊名: CHINESE JOURNAL OF VETERINARY SCIENCE
ISSN: 1005-4545
卷号: 001
基金: ^A河北省科技计划项目^B06220402D-2^D1
页码: 123-127
分类号: S857.2
摘要: 利用基因测序技术对CXCR2基因进行了多态性筛查,结果发现存在+612A→G、+684G→A、+777G→C、+855G→A、+858C→A和+861G→A等6个突变位点,其中+855为新发现的SNP.6个位点都处于连锁不平衡状态,+684与+861位点之间和+777与+858之间的相关程度高(R~2分剐为0.635和0.541).基因效应模型分析表明:+855和+861住点不同基因型对奶牛体细胞评分的影响没有显著性差异(P>0.05).+612位点不同基因型对体细胞评分的影响具有显著性差异(P<0.05),AA基因型个体的体细胞评分最低为4.19,乳房炎抗性最强.+777位点不同基因型对体细胞评分的影响差异性显著(P<0.05),GG基因型个体体细胞评分最低为4.17,对乳房炎的抗性较强,说明该基因+612和+777SNPS可作为奶牛乳房炎抗性分子标记位点.
英文摘要: DNA sequencing technology were used to detect the polymorphism of CXCR2 gene,results showed that, there were six polymorphism loci called+612A→G, +684G→A, +777G→C, + 855G→A, +858C→A and +861G →A in the amplified segment,and +855 SNP was the new discovery. Linkage disequilibrium analysis results showed that the six SNPs were mutually independent in the heredity. The degree of correlation with + 684 and + 861, + 777 and +858 were high so they can represent each other well. Linear modle analysis indicated:the two loci +855 and +861 had no significant effects on SCS(P>0.05). Different genotypes in +612 had significant effect on SCS(P<0.05) ,SCS LSM of AA genotype individuals had the lowest SCS 4.19 and better mastitis resistance. Different geno-types in +777 locus had significant effect on SCS(P<0.05),SCS LSM of GG genotype was 4. 17 and had relative mastitis resistance. +612 and +777 SNPs can be used as the molecular markers for mastitis resistance.