首页>>学术检索

复合酶协同微波处理提取紫背天葵叶片总黄酮工艺

标题: 复合酶协同微波处理提取紫背天葵叶片总黄酮工艺
作者: 崔蕊静,申淑琦,郭朔
出版时间: 2014-01-01
机构: 河北农业大学
关键词: 紫背天葵叶,复合酶,微波,提取,总黄酮
英文关键词: Begonia fimbristipula Hance leaves,complex enzyme,microwave,extraction,total flavonoids
刊名: 中国食品学报
英文刊名: Journal of Chinese Institute of Food Science and Technology
ISSN: 1009-7848
卷号: 002
页码: 72-77
分类号: TQ914
摘要: 目的:以紫背天葵叶片为原料,研究复合酶(纤维素酶和果胶酶)协同微波处理提取紫背天葵叶片中黄酮类物质,以及每个叶期不同叶片中黄酮类物质提取的工艺条件.方法:采用单因素及正交试验对复合酶水解后微波提取与直接微波提取总黄酮的效果进行对比试验.结果:将紫背天葵叶片50℃烘干,粉碎过80目筛,料水比1∶15(W∶V),加入0.2%果胶酶和0.05%纤维素酶,55℃水解40 min,再加入体积分数60%乙醇作提取剂,料液比1∶30,微波功率360 W,温度70℃下处理20 min后,所得浸提液中总黄酮含量比直接微波处理提高近30%.紫背天葵不同叶期、不同叶片黄酮类物质含量的规律为展开叶和成熟叶中总黄酮含量较高,幼叶次之,老叶中最低.结论:优化的工艺简便可行,提取率高,为紫背天葵黄酮类物质提取工艺的产业化提供理论依据.
英文摘要: Objective: With the Gynura bicolor leaves as raw material, to study on the composite enzyme(cellulose enzyme and pectinase) collaborative microwave processing to extract flavonoids from Gynura bicolor leaves, and the process conditions of flavonoids from leaves of different leaf in each period. Method: Using single factor and orthogonal test to contrast the effect of microwave extraction of flavonoids after composite enzyme hydrolysis and microwave extraction of total flavonoids directly. Results: The conte...