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切花菊高效不定芽再生体系的建立

标题: 切花菊高效不定芽再生体系的建立
英文标题: Efficient shoots regeneration from adventitious buds of cutting chrysanthemum
作者: 刘冰,杨际双,肖建忠,黄兆平,李单
英文作者: LIU Bing,YANG Ji-shuang,XIAO Jian-zhong,HUANG Zhao-ping,Li Dan
出版时间: 2011-01-01
机构: 河北农业大学,河北农业大学
关键词: 切花菊,不定芽,再生,叶盘
英文关键词: cutting chrysanthemum,adventitious buds,regeneration,leaf discs
刊名: 河北农业大学学报
英文刊名: JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI
ISSN: 1000-1573
期号: 34
卷号: 001
基金: ^A河北农业大学留学回国人员科研启动基金^B2004-897^D1
页码: 25-29
分类号: S682.1+1
摘要: 以"神马"和"出水芙蓉"2个切花菊品种的叶片为外植体,来建立不定芽再生体系.结果表明:基因型和植物生长调节剂是影响叶片不定芽再生的重要因素,并且较高浓度生长调节剂会导致不定芽和再生小苗玻璃化,不利于植株再生."神马"的最适直接诱导不定芽再生培养基为MS+NAA 1 mg/L+6-BA 1 mg/L,再生率高达955%;"出水芙蓉"的最适直接诱导不定芽再生培养基为MS+NAA 01 mg/L+6-BA 1 mg/L,但再生率仅为463%.植物生长调节剂影响再生植株生根数,适宜的生根培养基为1/2 MS+07 mg/L NAA.
英文摘要: Using leaf disks of cutting chrysanthemum(Jinba and Chushui Furong)as explants,an efficient regeneration system from the adventitious buds was established.The results indicated that the genotypes and plant growth regulators greatly affected shoot regeneration from adventitious buds.High concentrations of the plant growth regulators increased the vitrification of adventitious buds and shoots from leaves,and then,decreased the shoot regeneration.The optimum medium which could directly induce shoot regeneratio...