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瓜果腐霉的液体发酵工艺及代谢产物活性测定

标题: 瓜果腐霉的液体发酵工艺及代谢产物活性测定
英文标题: Liquid fermentation of Pythium aphanidermatum and bioassay of the metabolites
作者: 张利辉,董金皋,时俊光
出版时间: 2010-01-01
机构: 河北农业大学
关键词: 瓜果腐霉,代谢产物,除草活性,发酵
英文关键词: Pythium aphanidermatum,metabolites,herbicidal activity,fermentation
刊名: 河北农业大学学报
英文刊名: Journal of Agricultural University of Hebei
ISSN: 1000-1573
卷号: 006
基金: ^A国家高技术研究发展计划(863计划)^B2006AA10A214^D1
页码: 110-114,122
分类号: S476.1
摘要: 本试验对具有除草活性的瓜果腐霉PA1-M1菌株进行了液体发酵工艺探讨,并对其发酵产物进行了除草活性和抑菌活性测定.结果表明:PA1-M1菌株以振荡方式、中酸性发酵为适宜,其培养滤液粗提物和菌丝粗提物对马唐根和芽的IC50分别为0.36 mg/mL、0.46 mg/mL和7.36 mg/mL、4.09 mg/mL.此外,培养滤液粗提物对小麦赤霉病菌表现出了一定程度的抑菌活性.
英文摘要: Liquid fermentation was researched on isolate PA1-M1 of Pythium aphanidermatum and the bioassay was tested to its metabolites.The results showed that when it was cultured in a vibrating way and with the initial pH≤7 in the PD medium,the herbicidal activity and the dry weight of mycelium could reach the highest.The IC50 of the culture filtrate extracts and the mycelium extracts to Digtaria sanguinealis was 0.36 mg/mL,0.46 mg/mL and 7.36 mg/mL,4.09 mg/mL,respectively,and the culture filtrate extracts also had...