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枣离体叶片不定芽的诱导

标题: 枣离体叶片不定芽的诱导
英文标题: Induction of adventitious buds from in vitro leaves in Chinese jujube
作者: 魏薇,韩晶,刘孟军,王玖瑞,代丽
英文作者: WEI Wei1,HAN Jing1,LIU Meng-jun2,WANG Jiu-rui3,DAI Li2(1.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China,2.Research Center of Chinese Jujube,3.College of Forestry,China)
出版时间: 2012-01-01
机构: 河北农业大学,河北农业大学,河北农业大学
关键词: 枣,叶片,不定芽,诱导
英文关键词: Chinese jujube,leaf,adventitious bud,regeneration
刊名: 河北农业大学学报
英文刊名: Journal of Agricultural University of Hebei
ISSN: 1000-1573
卷号: 006
基金: ^A国家科技支撑计划项目^B^D1
页码: 51-54
分类号: S665.1
摘要: 以'月光枣'、'冬枣'和'木枣'为材料,利用培养基MS+麦芽糖20g/L+琼脂5g/L+TDZ 1.0mg/L+IBA 0.1mg/L+AgNO31.0mg/L,对枣树离体叶片不定芽进行诱导.结果表明:3个品种离体叶片出愈率均达到100%.芽分化培养基附加不同GA3、BA、TDZ时,不定芽发生情况差异明显.培养基MS+蔗糖30g/L+琼脂5g/L+IBA 0.2mg/L+6-BA 0.8~1.2mg/L适宜诱导'月光枣'叶片不定芽,出芽率和平均出芽数分别可达93.3%和3.53个,且无玻璃化现象.在培养基MS+蔗糖30g/L+琼脂5g/L+IBA 0.2mg/L+6-BA 0.8mg/L上,'冬枣'和'月光枣'叶片不定芽出芽率显著高于'木枣',2个品种出芽率均超过了93%,而'木枣'仅为66.63%.此外,附加乙酰丁香酮100mg/L可显著促进'冬枣'叶片不定芽发生,平均出芽数可高达7.30个.
英文摘要: Efficient induction of adventitious buds from in vitro Chinese jujube leaves was achieved using Ziziphus jujuba Mill.cv.'Yueguangzao','Dongzao' and 'Muzao'.Callus occurred in 100% of leaves from three cultivars cultured in medium MS+maltose 20 g/L+agar 5 g/L+TDZ 1.0 mg/L+IBA 0.1 mg/L+AgNO3 1.0 mg/L.The adventitious buds rate obviously changed in differentiation mediums separately supplemented with GA3,BA,TDZ.The medium MS+ sugar 30 g/L+ agar 5 g/L+ IBA 0.2 mg/L+6-BA 0.8-1.2 mg/L was appropriate for adventit...