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拟南芥抗灰霉病相关基因的克隆与原核表达

标题: 拟南芥抗灰霉病相关基因的克隆与原核表达
英文标题: Cloning and prokaryotic expression of resistant-related gene of Arabidopsis against Botrytis cinerea
作者: 陈展,郝丛丛,李莉,邢继红,董金皋
出版时间: 2010-01-01
机构: 河北农业大学
关键词: 拟南芥,灰葡萄孢,RACE,原核表达
英文关键词: Arabidopsis,Botrytis cinerea,RACE,prokaryotic expression
刊名: 河北农业大学学报
英文刊名: Journal of Agricultural University of Hebei
ISSN: 1000-1573
卷号: 005
基金: ^A河北省自然科学基金^B08B021^D1%^A河北农业大学9816科技重点项目资助^B^D2
页码: 29-33
分类号: S432.2
摘要: 为了从拟南芥中获得抗灰霉病相关基因以及进行基因分析,本试验利用RACE技术获得了该基因的cDNA全长(1190 bp),并用生物信息学方法和Southern Blotting技术明确了该基因是编码372个氨基酸的转录调节/结合蛋白的AT5G67480基因,以单拷贝形式存在于拟南芥基因组中.编码的蛋白含有BTB/POZ结构域,体外诱导表达的蛋白对灰霉病菌的生长有一定的抑制作用.
英文摘要: Full-length cDNA sequence of resistant-related gene of Arabidopsis against Botrytis cinerea was obtained by running RACE.Result of homology indicated that gene identified as AT5G67480 gene,which encoded binding protein or transcription regulator,containing 372 amino acids.AT5G67480 gene is a single copy in Arabidopsis genomic DNA by Southern Blotting analysis.The protein encodes by AT5G67480 gene contains BTB/POZ domain of TAZ zinc protein family.In most cases,protein containing BTB/POZ domain is related to...