首页>>学术检索

银杏过氧化氢酶基因CAT1的克隆及表达分析

标题: 银杏过氧化氢酶基因CAT1的克隆及表达分析
英文标题: Molecular Cloning,Characterization and Expression of Catalase1 Gene from Ginkgo biloba
作者: 程华,李琳玲,许锋,王燕,程水源
出版时间: 2010-01-01
机构: 河北农业大学
关键词: 银杏,CAT1基因,表达分析
英文关键词: Ginkgo biloba.,Catalase1 gene,expression analysis
刊名: 林业科学研究
英文刊名: Forest Research
ISSN: 1001-1498
卷号: 004
基金: ^A教育部新世纪优秀人才支持计划项目^BNCET-04-0746^D1%^A湖北省教育厅重大科技项目^BZ200627002^D2%^A湖北省青年杰出人才基金^B2003AB014^D3
页码: 493-499
分类号: S792.95
摘要: 利用RACE技术从银杏中克隆到过氧化氢酶基因(GbCAT1)的cDNA全长.进化树分析结果表明:GbCAT1和其他物种的CAT源自于相同的祖先.Southern 杂交显示:GbCAT1属于1个小的多基因家族.实时定量RT-PCR分析表明:GbCAT1在银杏的根、茎、叶和果中都有表达,在叶中的相对表达量最高,其次为果、茎和根.GbCAT1的转录受到ABA、渗透压、紫外、低温和高温胁迫的诱导.水杨酸处理下,GbCAT1相对表达量迅速降低.CAT1基因在逆境条件下的相对表达变化与环境胁迫有关.
英文摘要: The full-length cDNA sequence of Ginkgo biloba L.Catalase1 gene was isolated from G.biloba.Phylogenetic tree analysis revealed that GbCAT1 shared the same ancestor with other CAT.The result of Southern analysis showed that GbCAT1 gene was encoded by a small gene family in G.biloba.The expression analysis by RT-PCR showed that GbCAT1 expressed in a tissue-specific manner in G.biloba.GbCAT1 was also found to be up-regulated by the four tested abiotic stress,abscisic acid,osmotic stress,low temperature,and the...