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衡水某奶牛场临床型乳房炎病原菌的分离鉴定及药敏试验

标题: 衡水某奶牛场临床型乳房炎病原菌的分离鉴定及药敏试验
作者: 杨建辉,秦建华,冯向辉,陈松,韩旭东,张爱民,李铁拴
召开时间: 2010-08-15
机构: 河北农业大学
关键词: 奶牛
英文关键词: dairy cow
会议名称: 中国畜牧兽医学会2010年学术年会-第二届中国兽医临床大会
会议地点: 中国吉林长春
主办单位: 中国畜牧兽医学会(Chinese Association of Animal Science and Veterinary Medicine)
分类号: S858.23
摘要: 无菌采集衡水市某奶牛场临床型乳房炎牛的乳样20份,进行了病原菌的分离培养及生化鉴定及药敏试验.从20份乳样中共分离到9种162株细菌,其中金黄色葡萄球菌58株(35.8%)、大肠杆菌58株(35.8%)、表皮葡萄球菌10株(6.2%)、腐生葡萄球菌6株(3.7%)、乳房链球菌10株(6.2%)、无乳链球菌8株(4.9%)、停乳链球菌10株(6.2%)、克雷伯氏菌1株(0.6%)、变形杆菌1株(0.6%).结果表明:葡萄球菌、大肠杆菌为主要病原菌,其次是链球菌,占总菌数的98.8%(160/162);药敏试验结果表明:主要致病菌对硫酸头孢喹诺注射液(M、G)、中药复方黄连乳腺炎注射液(M)、林可霉素等5种药物呈高度敏感.
英文摘要: 20 milk samples of clinical dairy cow mastitis were collected from a dairy cow farm of Hengshui area, and the isolation,culture and biochemical identification of pathogenic bacteria,and drug susceptibility test were made.The results showed that 162 bacteria and 9 species were isolated from 20 samples,including 58 Staphylococ-cus aureus(35.8%),58 Escherichia Coli(35.8%),10 Staphylococcus epidermidis(6.2%),6 Staphylococcus saprop-hyticus(3.7%),10 Streptococcus uberis(6.2%),8 Streptococcus mastitidis(4.9%),10 Streptococcus dysgalactia-e(6.2%),1 Klebsiella(0.6%),1 Bacillus termo(0.6%).Staphylococci,Escherichia coli and Streptococcus were the main bacteria for causing cow clinical mastitis.The main pathogens were Staphylococcus and Streptococcus accounted for 98.8%(160/162).The drug susceptibility test showed that the most sensitive antibiotics to pathogenic bacteria were Cefquinome sulfate,compound recipe of Huanglian, Lincomycin,Ceftriaxone and Neomycin sulfate.