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麻杏石甘粉对雏鸡支气管IgA分泌细胞的影响

标题: 麻杏石甘粉对雏鸡支气管IgA分泌细胞的影响
作者: 刘鹏飞,钟秀会
召开时间: 2010-08-15
机构: 河北农业大学
关键词: 麻杏石甘粉
英文关键词: Maxingshigan powder
会议名称: 中国畜牧兽医学会2010年学术年会-第二届中国兽医临床大会
会议地点: 中国吉林长春
主办单位: 中国畜牧兽医学会(Chinese Association of Animal Science and Veterinary Medicine)
分类号: S858.31
摘要: 将麻杏石甘粉分别按照0.5g/L、1g/L、1.5g/L溶于水后投喂给人工感染传染性支气管炎病毒的15日龄雏鸡,连续3天.给药后观察其临床疗效,分别在18、21、24、27、30日龄时剖杀,摘取气管分叉处,免疫组织化学方法测其单位面积IgA分泌细胞的变化,同时通过ELISA方法测定血清中IBV抗体滴度.临床疗效结果表明,麻杏石甘散能显著降低病鸡的死亡率,提高对其的治愈率.在体内能显著提高雏鸡支气管IgA分泌细胞的阳性表达率及血清中IBV抗体滴度,提示麻杏石甘散能有效保护IBV雏鸡的黏膜,促进产生IgA分泌细胞,并且能促进机体产生抗体,从而达到治疗作用.
英文摘要: In this paper the effects of Maxingshigan powder were investigated on IgA-secreting cells of chicken bronchus.The powder was dissolved in water at three dosages,0.5g/L,lg/L and 1.5g/L.Each dosage was given to 15-day-old chickens which were infected with infectious bronchitis virus by artificial infection for 3 days.And then the therapeutic effect was observed.The chickens were sacrificed at 18,21,24,27 and 30 days old.The changes of IgA-secreting cells per unit area of bronchus were measured by immunohistochemistry.The IBV antibody titers in serum were determined by ELISA.The results of the therapeutic effect showed that Maxingshigan powder can significantly reduce the mortality and enhance the recovery rate.Positive expression rate of bronchial IgA-secreting cells and higher IBV antibody titers were observed in the herbal treat groups.The results suggest that Maxingshigan powder can protect the mucosa of IBV chickens,promote the mucosa producing IgA secreting cells and also the IBV antibody so as to lower the mortality and elevate the recovery rate of the IBV chickens.