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花生胚小叶对卡那霉素的敏感性研究

标题: 花生胚小叶对卡那霉素的敏感性研究
英文标题: Study on Sensitivity of Peanut Leaflet to Kanamycin
作者: 王凤欢,何美敬,杨鑫雷,崔顺立,穆国俊,侯名语,刘立峰
英文作者: WANG Feng-huan,HE Mei-jing,YANG Xin-lei,CUI Shun-li,MU Guo-jun,HOU Ming-yu,LIU Li-feng,North China Laboratory of Crop Germplasm Resources of Education Ministry,Key Lab.for Crop Germplasm Resources of Hebei,College of Agronomy,Agr.Univ.of Hebei
出版时间: 2016-05-24
机构: 教育部华北作物种质资源实验室/河北省作物种质资源重点实验室/河北农业大学农学院
关键词: 花生,卡那霉素,基因型,敏感性
英文关键词: peanut(Arachis hypogaea L,),kanamycin,genotype,susceptibility
刊名: 花生学报
英文刊名: Journal of Peanut Science
ISSN: 1002-4093
期号: 02
卷号: v.45
国内刊号: 37-1366/S
基金: 国家自然科学基金(31471523); 农业部引进国际先进农业科学技术计划(“948”计划)(2013-Z65); 高等学校博士学科点专项科研基金(2012130211002); 河北省高等院校科学技术研究重点项目(ZH2011209)
主题: 花生胚小叶对卡那霉素的敏感性研究
页码: 17-22+34
分类号: S565.2
出版单位: 花生学报
是否SCI: 0
是否EI: 0
是否核心: 0
摘要: 卡那霉素是植物遗传转化中常用的一种筛选剂。研究花生胚小叶对卡那霉素的敏感性,对建立利用卡那霉素筛选花生遗传转化的有效体系具有重要意义。以3个不同基因型花生弗落蔓生、麻油1-1和濮花23号为试验材料,通过计算胚小叶黄化率、丛生芽诱导率和生根数等,并观察外植体的生长状态,研究不同浓度卡那霉素对胚小叶生长发育、丛生芽分化和丛生芽生根阶段的影响,以期确定3个品种胚小叶各个分化阶段的适宜筛选浓度。结果表明,不同基因型花生对卡那霉素的敏感性不同,弗落蔓生、麻油1-1和濮花23号胚小叶丛生芽分化筛选浓度依次为:150mg/L、100mg/L和50mg/L;丛生芽生根筛选浓度分别为:弗落蔓生20mg/L、麻油1-1 20mg/L和濮花23号10mg/L。本研究筛选到不同品种胚小叶丛生芽分化和丛生芽生根阶段的适宜卡那霉素浓度,为以花生胚小叶为外植体的花生遗传转化阳性植株的筛选奠定了基础。
英文摘要: Kanamycin is a screening agent commonly used in plant genetic transformation.The study of susceptibility of peanut leaflet to kanamycin is important to the genetic transformation of peanut.3different genotypes of peanut(Fuluomansheng,Mayou1-1and Puhua23)were used to study the impact of different concentration of kanamycinon on the development,differentiation and rooting of clustered shoots of peanut leaflet by calculating their yellowing rate,clustered shoots induction rate,rooting percentage and observing the growing status of explants,so as to confirm the suitable screening concentration.The results showed that different genotypes of peanut had different degrees of susceptibility to kanamycin.The suitable concentration of kanamycin for the differentiation of leaflet clustered shoots was 150mg/L for Fuluomansheng,100mg/L for Mayou1-1and 50mg/L for Puhua 23;while for rooting of clustered shoots,the suitable concentration of kanamycin was 20mg/L for Fuluomansheng,20mg/L for Mayou1-1and 10mg/L for Puhua 23.In this study,suitable screening concentration of kanamycin in stages of differentiation and rooting of clustered shoots was obtained,which laid foundation for the screening of positive plant in genetic transformation of peanut leaflet.